The route is a loop along the Biodiversity Trail at the foot of Mount Grignone near Esino Lario in the Alto Lario Lecchese. Esino Lario can be reached from the lake by going up from Varenna and Perledo along the Strada per Esino, or from Valsassina along SP65 Cortenova - Perledo, passing through the magnificent viewpoint of Passo di Agueglio. Free parking lots are on Via alle scuole, Viale Adamello and Via Bertarini near the Grigne Museum. The start is from the central square of Esino Lario. From here, start climbing in the direction of Agueglio Pass, then turn onto the dirt path marked as the "Biodiversity Trail" along State Road 753 in Esino. Continue along a forest road halfway up the hillside until you reach the village of Cainallo. This section has small climbs and descents, with some particularly challenging sections over uneven terrain. Upon reaching Cainallo, it is possible to make a brief stop to admire the surrounding landscape at the Cainallo Refuge. From Cainallo, the route continues in the direction of St. Peter's Church in Ortanella, following technically accessible but physically demanding forest roads and trails. The surrounding environment is typically pre-Alpine, immersed in a beautiful beech forest. From Ortanella, return to Esino passing through the valley of the Esino and Busagna streams, following forest roads that are particularly steep in some sections. It is possible to take a break and recharge electric bikes at Agriturismo Ortanella or at Agriturismo dei cacciatori.